Total RNA purification is a fundamental step in numerous molecular biology experiments. This process requires the careful separation of total RNA from diverse biological sources, such as tissues, cells, and body fluids. The objective is to check here obtain high-quality RNA that is undegraded from contaminating molecules. A variety of methods are
Isolation and Isolation of RNA
RNA is a fragile molecule that is easily degraded by enzymes called RNases. Therefore, it is essential to precisely handle RNA samples and use sterile techniques during isolation and purification processes. There are several methods available for isolating RNA from various tissues, including {phenol-chloroform extraction, column chromatography, and